2018-8-22

D/T undoubted residual Cryptobia chronic infection in the 210G discus tank and the introduction of a new healthy discus from NADA meeting, I will treat the current discus stock/tank with Dimetridazole to help decrease the risk of the new fish being infected.  Previous introductions to this tank have resulted in 75% mortality with confirmed Cryptobia.  Interestingly, the Raffelesia discus have been resistant to any signs of Cryptobia infection.

210gallons + 50 gallon sump= about 1000L

dimetridazole 80mg/L=> 80gm

This is difficult to sustain at this volume, but capturing discus in this planted tank will be difficult.  I'm considering options.

2018-6-4

Sam's discus fish showing wasting disease.  I failed to QT them.

3 fish dead.  New fish won't even eat live black worms.

Per http://edis.ifas.ufl.edu/vm077  

a bath treatment with dimetridazole (80 mg/L for 24 hrs,
followed by a 80-100% water change, repeated daily for 3
days); or b) a bath treatment with 2-amino-5-nitrothiazol
(10 mg/L for 24 hrs, followed by a 80-100% water change,
repeated daily for 3 days).

240 gallons=800 L

Treated

also with TCN, Rid-Ich, Melafix....the kitchen sink.

2018-3-6

Small
Serra nitrate 50
JBL nitrate 40

Have not added nitrate in a week.  More food and more fish are the reason.



Lg
Water change

2017-10-10 New discus not eating well

Received these discus from Kenny  on 8/21 and placed in 40 gallon QT tank.

On 8/11/2017 4:21 PM, Carey Cottle MD wrote:

I’ll take the

5pcs 3.5"+/- Fiamma Rossa--$75 *from strain creator Ung Seng Lip
2pcs 4"+/- Rafflesia--$115 (nice red mix with Fiamma Rossa).

The fish have a poor appettite and I have seen stringy poop at time.  One stool analysis did not reveal cryptobia. But I suspect it.  

1.Will raise the temp to 92F and aggressively treat with 

2. flubendazole (1/2 gram of 10% Flubendazole per 20

gallons).=1g/40gallons

3.Metronidazole 2g/40gallons: Metro is best administered at 92F +/- so initially raise the tank temp to be around that range, and dose 500mg per 10g(Metro from the above link is at 250mg strength so use 2 tablets per 10g) and re-dose every 8-12 hours with a full single dose, with a 50% daily WC for a total of 5-7 days. Upon finishing the Metro treatment I would keep the tank temp elevated at around 90F for another week just to maintain their metabolic rate higher for a little longer.

Btw you should pre-dissolve the Metro tablets/powder(calculate how many Metro you'll need to use for your tank size beforehand if using tablets) with hot water in a small container and shake them well, prior to pouring them in to your main tank.

Sincerely,

Kenny Cheung
Kenny's Discus

4. 2-amino-5-nitrothiazol=10mg/L in 40gallons or 150Liters=1500mg.

Per http://edis.ifas.ufl.edu/vm077  

a bath treatment with dimetridazole (80 mg/L for 24 hrs,
followed by a 80-100% water change, repeated daily for 3
days); or b) a bath treatment with 2-amino-5-nitrothiazol
(10 mg/L for 24 hrs, followed by a 80-100% water change,
repeated daily for 3 days).

2015-6-15 Crytpobia Discus plague dosing strategy

Carey,

Thanks for the update-I’m glad to hear that something is working!. Since the cryptobia are naturally found in the discus, I don’t know if eradication is feasible. Higher dosing in food materials may be a possible way to eliminate the infection.



From: Cottle, Carey
Sent: Friday, June 12, 2015 12:46 PM
To: Baumgartner, Wes; Dr. Sam Young

Subject: Cryptobia update

Drs.,

My first attempt at using dimetridazole was inadequate bc I underdosed, not taking into account the powder was 40% active ingredient.  I redosed and the fish have responded well.  Off the med for 2 weeks fish are eating well and regaining weight.  My interpretation of the appearance of the stool with presence of fat globules and other poorly digested food suggests some malabsorption is still present probably from prior damage to the GI tract.  There are still a few Cryptobia evident.  At 100X there are at most 3 per field of view.  Must I fully eliminate the Cryptobia to prevent disease?  At present the fish are not symptomatic except for being a bit underweight with the evidence of malabsorption in one of the 2 fish.

BTW, after using the messy product Emtryl for the source of dimetridazole I found that a Marcel product for fish called Clout contains this without the messy additives that cloud the water.

I think I will try this as recommended in the Cryptobia article from IFAS.  FYI, I got this from Sigma Aldrige chemical company and it was not easy to obtain.

bath treatment with 2-amino-5-nitrothiazol
(10 mg/L for 24 hrs, followed by a 80-100% water change,
repeated daily for 3 days).


I spoke with Dr. Chen in Washington state about his study using isometamidium to treat Cryptobia in Salmon and he was encouraging.  However, it has been impossible to obtain this in the US so far.

If you have other suggestions, let me know.

Thanks for your interest and advice,

2013-4-7

2015-5-15

Sorry, Greg/Dan- and Carey- I think I meant “he’s more local”?

Roy

__________________________________________________________________________________________________________________________________
 Roy P. E. Yanong, VMD  |  Professor  |  Extension  Veterinarian
 Tropical Aquaculture Laboratory  |  Program in Fisheries and Aquatic Sciences  |   School of Forest Resources and Conservation  |  University of Florida / IFAS
 1408 24th St. SE, Ruskin, FL  33570  |  813-671-5230 x 104  |  Fax: 813-671-5234   |  rpy@ufl.edu
 Tropical Aquaculture Laboratory:  http://tal.ifas.ufl.edu   |   Fisheries and Aquatic Sciences: http://sfrc.ufl.edu/fish/  |
 Aquariumania podcast: http://petliferadio.com/aquariumania.html
________________________________________

From: Gregory Lewbart [mailto:galewbar@ncsu.edu]
Sent: Tuesday, May 05, 2015 1:49 PM
To: Yanong, Roy P.E.
Cc: Carey Cottle Jr MD; Gregory A. Lewbart; drdan@avianandexotic.com; drexotic2@hotmail.com; VHC Exotic Medicine
Subject: Re: I would like to consult you regarding cryptobia infection in discus

Dear Dr. Cottle,
I'm sure both Dr. Johnson and I would be happy to try and help.  I see cases through our Exotic Animal Medicine Service (EAMS) here at the CVM:

http://www.cvm.ncsu.edu/vhc/vhwc/exotics/index.html
To make an appointment you can call 919-513-6999.
Dr. Dan runs a large exotic animal medicine practice and is an adjunct professor at the CVM:

http://avianandexotic.com/
Thanks Roy and best to all,
Greg

On Tue, May 5, 2015 at 12:25 PM, Yanong, Roy P.E. <rpy@ufl.edu> wrote:
Hey, Carey-
Sure, I’d love to help, but I think it would make more sense for you to be in touch with someone in NC.  Dr. Greg Lewbart and Dr. Dan Johnson (both cc’d on this email), two veterinary colleagues of mine who have a lot of experience with fish medicine (they are both excellent clnicians!) should be able to help you out.  Please let me know if/when they get in touch with you (or I’ll send a reminder (!)).

Greg and Dan-
Can one of you help Carey out?  She’s more local to you.

Roy

__________________________________________________________________________________________________________________________________
 Roy P. E. Yanong, VMD  |  Professor  |  Extension  Veterinarian
 Tropical Aquaculture Laboratory  |  Program in Fisheries and Aquatic Sciences  |   School of Forest Resources and Conservation  |  University of Florida / IFAS
 1408 24th St. SE, Ruskin, FL  33570  |  813-671-5230 x 104  |  Fax: 813-671-5234   |  rpy@ufl.edu
 Tropical Aquaculture Laboratory:  http://tal.ifas.ufl.edu   |   Fisheries and Aquatic Sciences: http://sfrc.ufl.edu/fish/  |
 Aquariumania podcast: http://petliferadio.com/aquariumania.html
________________________________________

From: Carey Cottle Jr MD [mailto:ccottle@earthlink.net]
Sent: Tuesday, May 05, 2015 12:13 PM
To: Yanong, Roy P.E.
Cc: ccottle@earthlink.net
Subject: I would like to consult you regarding cryptobia infection in discus

Dr. Yanong,

I’m in NC but would like to consult you and pay for your services in helping me with my discus.  I’ve consulted a vet here, Dr. Sam Young but he’s not an expert in fish.

My discus have a chronic wasting disease.  Three fish have been necropsied and I can send those reports to you if you wish.  They show stomach and other areas of granulomas that were negative for acid fast staining.  Dr. Wes Baumgartner suggested the most likely diagnosis was Cryptobia.  I was able to ID cryptobia in a stool sample once I knew what to look for.  I followed his suggestion to try dimetridazole (Emtryl) at 100mg/L.  I put the 2 discus who’d stopped eating in a hospital tank and used the bath for 7 days with temps raised in the tank to 90F and pH lowered gradually to 4.0 for the antibacterial effect.  Low pH does not kill Cryptobia, but the fish began eating again as soon as the pH dropped to 4.0.  The Cryptobia is gone from the last 2 stool samples after the dimetridazole bath.

However, I now have another larger parasite in the stool sample.  I don’t know what it is but it is about the size of a monogean.  I would like to get you to id it for me.  I can send a video is you are willing to help.

Thanks for your time,

Carey Cottle MD



--
Gregory A. Lewbart  MS, VMD, Dipl. ACZM
Professor of Aquatic Animal Medicine
North Carolina State University
College of Veterinary Medicine
1060 William Moore Drive
Raleigh, NC  27607
greg_lewbart@ncsu.edu

http://www.cvm.ncsu.edu/vhc/vhwc/exotics/index.html
http://marinesci.ncsu.edu/